Biodata Profile Sample Submission

Prerequisites & Notes

Important

Note

Samples cannot be deleted after they have been submitted.


Accessing the Samples Page

The Samples page can be accessed via the Components button or via the components icon navigation pane when viewing another component’s page associated with a Biodata profile.

Using the Components Button

Click the samples-component-button component button in the Components column as shown below:

Samples profile component button

Samples component button (located under Biodata profiles)


Using the Components Icon Navigation Pane

The component icon navigation pane provides a quick access to other components associated with the same profile. It appears on the inner right pane of the screen when you are viewing a component’s page. The icons displayed in the navigation pane correspond to the components associated with the profile.

If you are unsure what each icon represents, you can perform any of the following:

  • Hover over the icon in the component icon navigation pane to see a tooltip with the component’s name

  • Refer to the component button image above

  • Refer to the What are profile components? FAQ

Note

  • The navigation pane will not display the icon associated with the page that is currently being viewed.

  • If a component has subcomponents, a dropdown menu is shown when at least two items are available. However, if you are viewing a subcomponent’s page and only one item remains, that item is displayed directly in the navigation pane.

Samples profile component icon

Navigation pane showing the Samples component icon


Upload Samples

  1. Click the dropdown menu to choose a checklist [3].

    Pointer to Samples checklist dropdown menu

    Pointer to dropdown menu for samples


    Hover over each option to view its description. An overview of each option is provided in the Sample manifest checklist section.


    Available samples checklist options within a Biodata profile

    Checklist options for sample submissions


  2. Click the samples-blank-manifest-download-button-biodata button to download a blank manifest [4].

    A manifest is a spreadsheet file used to record metadata for submission.

  3. Fill in the downloaded manifest then, click the add-samples-manifest-button-biodata button to upload it from your local (computer) system.

    Note

    Please ensure that the manifest that you are uploading matches the checklist type selected in step 1. You will encounter errors if the uploaded manifest does not correspond with the selected dropdown menu checklist option.

    Pointer to 'Add or update samples from spreadsheet' button

    Click “Add or update samples from spreadsheet” button to open an upload dialogue


  4. A dialogue is displayed. Click the samples-upload-button-biodata button in the dialogue to choose the spreadsheet file from your local system.

    Upload Samples spreadsheet dialogue

    Click ‘Upload sample manifest’ button


  5. The uploaded data is shown in a preview before final submission. Click the samples-finish-button button to finalise the upload.

    Dialogue with uploaded Samples data

    Samples upload preview


  6. The new samples data will be displayed on the Samples page after a successful validation.

    Samples data uploaded

    Samples page showing the uploaded data


Submit Samples

Note

  • Follow the steps in Upload Samples to upload data before proceeding with the submission.

  • The following repositories are supported for Samples submissions:

    • European Nucleotide Archive (ENA) via submit-record-button-ena button

  1. After uploading data, select a row in the data table.

    Then, click the submit-record-button-ena button to submit the selected record to ENA.

    Hint

    Selected rows are indicated by a blue background colour.

    Note

    Please ensure that the selected record is under the desired checklist option. If not, choose another option from the dropdown menu, then select a row in the data table.


  2. Wait for the submission process to be completed. The submitted record’s status and accessions can be viewed in the data table’s respective columns. See the sections below for details.

Make Data Public

  1. Sample submissions are automatically made public after they have been submitted. You can therefore access the data publicly in the associated repository after a couple of days of processing. via the accessions.

    See the View Accessions section below for more details.


View Submission Status

Hint

The Data status & progress legend, located on the right side of the data table, shows the different stages of submission processing. Hover over the info-icon to see description for each status.

For more details, see the How can I check the status of my data submissions? FAQ section.

The submission will be scheduled for processing after the submit-record-button-ena button is clicked. The row of the submitted record will be highlighted in green indicating that the submission has been successful.

If the submission was unsuccessful or has not been assigned any accessions, the table row will be highlighted in red. Its status is indicated in the following columns:

  • STATUS - Displays the status returned from the repository, European Nucleotide Archive (ENA), assigned to the project

  • ERROR - Displays any error message associated with the submission.


View Accessions

Accessions are unique identifiers assigned to submissions after they have been successfully submitted to public repositories.

The accession columns are:

  • BIOSAMPLEACCESSION - the biosample accession is the primary identifier for the sample submission.

  • SRAACCESSION - the SRA [5] accession is the secondary identifier for the sample submission.

Clicking any accession in the data table will open the corresponding record in the associated repository. You can also find the same record by searching for the accession directly in the repository.

Tip

Alternatively, click the accessions-component-icon icon located in the top-right corner of the data table to navigate to the Accessions page that displays accessions for all submissions.